蚕蛹中蛋白质与甲壳分离的研究

以脱脂蛹为原料,采用电子光学显微分析技术,观察酸、碱浸泡样组织结构变化,确定了碱法为蛋白质与甲壳素的分离方法。选定了NaOH溶液为分离试剂,就工艺条件(温度、碱浓度、固液比、时间)对蚕蛹蛋白与甲壳素分离效果的影响进行了研究。结果表明,在本实验条件下,最佳工艺条件:温度80℃,固液比1:12,时间10min,碱浓度1(wt)%。甲壳得率为2.46%,粗蛋白回收得率为78.91%。

Research on separation of proteins and chitins in silkworm chrysalis

In this paper,taking defatted silkworm chrysalis as raw material and using microscopic analysis technology of electronics and optics,we observed the change of structure of the sample which was soaked in hydro- chloric acid solution and sodium hydroxide solution,and ascertained the alkaline approach as the method of sepa- rating proteins and the chitins.Furthermore,according to the effect of technological conditions(alkali concentra- tion,temperature,ratio of solid and liquid,time)on separation of proteins and chitins,we selected sodalye a- queous alkali for further studying.The result showed,that under the experiment condition,optimum technological conditions was:80 centigrade,1(wt)% as alkali concentration 1:12 as solid to liquid and 10 mininutes.The at- tainable rate of chitins was 2.46%,the reclaimable rate of proteins was 78.91%.