Surface plasmon resonance immunosensor using Au nanoparticle for detection of TNT |
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Authors: | Toshikazu Kawaguchi Dhesingh Ravi Shankaran Sook Jin Kim Kiyoshi Matsumoto Kiyoshi Toko Norio Miura |
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Affiliation: | aArt, Science and Technology Center for Cooperative Research, Kasuga-shi, Fukuoka 816-8580, Japan;bJapan Society for the Promotion of Science, Tokyo 102-8471, Japan;cGraduate School of Agriculture, Kyushu University, Fukuoka 812-8581, Japan;dGraduate School of Information Science and Electrical Engineering, Kyushu University, Fukuoka 819-0395, Japan |
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Abstract: | In order to develop the fully integrated portable surface plasmon resonance (SPR) system for detection of explosives, the amplification strategy of SPR signal was investigated. Indirect competitive inhibition method allowed the middle-sized SPR sensor to detect trinitrotoluene (TNT) at ppt level. However, this enhanced SPR signal was not high enough to detect TNT at ppt level by a miniaturized SPR sensor. Therefore, localized surface plasmon resonance (LSPR) effect using Au nanoparticle as further signal amplification approach was used. The amplification method of indirect competitive inhibition and LSPR were combined together for fabrication of the immunosurface using Au nanoparticle. TNT detectable range of this immunosurface was from 10 ppt (10 pg/ml) to 100 ppb (100 ng/ml), which was almost comparable to that without Au nanoparticle. The observed resonance angle change due to binding monoclonal TNT antibody (M-TNT Ab) with the immunosurface modified with Au nanoparticle was amplified to four times higher than that in absence of Au nanoparticle. |
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Keywords: | Localized surface plasmon resonance (LSPR) Au nanoparticle TNT SPR Immunosensor Monoclonal anti-TNT antibody |
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