广东淮山水溶性多糖的分离纯化及体外抗氧化活性的研究

广东淮山经热水提取、乙醇沉淀得粗多糖，再经脱脂、脱蛋白、脱色、两次Sephadex G-75柱层析纯化得多糖精品，经纸层析、旋光度测定、紫外光谱鉴定为均一多糖。在体外设计产生超氧阴离子自由基、羟基自由基和脂质自由基等3个体系，分别加入广东淮山不同浓度的多糖粗品和精品，结果表明，广东淮山多糖粗品及精品对超氧阴离子自由基和羟自由基均有较高清除作用，但对脂质自由基的清除能力较弱，而且经纯化精制后的广东淮山多糖对超氧阴离子自由基和羟自由基的清除作用均小于其粗品。

Study on the Isolation, Purification and Anfioxidative Actvity of Water Soluble Polysaccharide of D. fordii Prain et Burkill

The crude polysaccharide was extracted from D. fordii Prain et Burkill with hot water,and precipitated by ethanol.The refined polysaccharide was prepared after delipid, deprotein and decolor processing with further purification on two timesSephadex G-75 column chromatography. The homogentity was examined with paper chromatography and the determination ofspecific rotatory power identified by ultraviolet spectra. In order to estimate the antioxidation activity of D. fordii Prain et Burkill,three systems of generating superoxide radical, hydroxyl radical and lipid radical respectively in vitro were designed. The resultsshowed that both crude and refined Polysaccharides from D. fordii Prain et Burkill were effective in scavenging superoxide radicaland hydroxyl radical, but only a little effective in scavenging lipid radical, and that the scavenging for capacity superoxide radicaland hydroxyl radical of refined polysaccharide extracted from D. fordii Prain et Burkill were higher than that of crudepolysaccharide alse extracted from D. fordii Prain et Burkill.