| 柱前衍生化-高效液相色谱法测定啤酒中的多胺 |
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| 引用本文： | 王利娜,金东日.柱前衍生化-高效液相色谱法测定啤酒中的多胺[J].延边大学理工学报,2014(3):249-252. |
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| 作者姓名： | 王利娜 金东日 |
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| 作者单位： | 延边大学理学院化学系,吉林延吉133002 |
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| 基金项目： | 国家自然科学基金资助项目（21365022） |
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| 摘 要： | 建立了用柱前衍生化高效液相色谱法分析啤酒中多胺的方法.在碱性介质中,多胺与1-(5-氟-2,4-二硝基苯基)-4-甲基哌嗪(PPZ)进行衍生化反应生成衍生物.实验采用Diamonsil C18(2)(迪马,150mm×4.6mm,3μm)色谱柱,以水-有机溶剂(乙腈-甲醇,体积比为6∶4)作为流动相,流速为0.5mL/min,用梯度洗脱程序方法同时分离5种多胺衍生物,衍生物的检测波长为362nm.通过考察方法的线性范围、稳定性、检测限和回收率表明:本方法具有样品处理简单、灵敏度高、速度快的优点,可用于啤酒中多胺含量的测定.
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| 关 键 词： | 高效液相色谱 多胺 1-(5-氟-2 4-二硝基苯基)-4-甲基哌嗪 |
Determination of polyamines in beer using high-performance liquid chromatography with derivatization reagent |
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| WANG Lina,JIN Dongri.Determination of polyamines in beer using high-performance liquid chromatography with derivatization reagent[J].Journal of Yanbian University (Natural Science),2014(3):249-252. |
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| Authors: | WANG Lina JIN Dongri |
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| Affiliation: | ( Department of Chemistry, College of Science, Yanbian University, Yanji 133002, China ) |
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| Abstract: | A high performale liquid chromatography method was developed for the determination of polyamines in beer. The polyamines were derivatived by 1-（5-fluoro-2, 4-dinitrophenly）-4-methlypiperazine （PPZ） in alkaline condition and then separated on a Diamonsil C18 （2） （Dikma, 150×mm 4.6mm×3μm） column. The effect of mobile phase composition was investigated at detection wavelength of 362 nm. The result indicated that the optimum separation was obtained by using water： （acetonitrile ： methanol= 6：4, v/v） as the mobile phase with gradient elution （flow rate of 0.5 mL/min）. The linear range, stability, detection limit and recovery were examined. The method was simple, sensitive and rapid. The proposed method has been applied to the determination of polyamines in beer. |
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| Keywords: | K high performale liquid chromatography polyamines 1-（ 5-fluoro-2 4-dinitrophenly）-4-meth-lypiperazine |
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