| 高压反相液相色谱法分析[~3H]CAMP与[~3M]CGMP |
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| 引用本文： | 吴叔筠,茅庆南,林志豪,赖运琅. 高压反相液相色谱法分析[~3H]CAMP与[~3M]CGMP[J]. 原子能科学技术, 1987, 0(2) |
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| 作者姓名： | 吴叔筠 茅庆南 林志豪 赖运琅 |
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| 作者单位： | 中国原子能科学研究院 北京(吴叔筠，茅庆南，林志豪)，中国原子能科学研究院 北京(赖运琅) |
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| 摘 要： | CAMP(环磷酸腺苷)和CGMP(环磷酸鸟苷)属环核苷酸类,,在机体中的含量极低,约为每mg湿组织pg分子水平,用一般的分析方法不能测定。主要采用竞争性蛋白质结合分析法、放射免疫法和薄层色谱法进行分析。近年来,用高压液相色谱分离分析环核苷酸,定量方便、分析速度快、检测装置灵敏度高,具有其他分析方法所没有的优点。过去,对环核苷酸的分离都在离子交换柱上进行,最近,Krstulovic等采用反相色谱,在μBondapak C_(18)柱上进行分离分析,不需对样品进行预浓缩,使方法更为简便,而且柱寿命长。[~3H]CAMP是竞争性蛋白质结合分析法测定环磷酸腺苷,以及[~3H]CGMP是放射免疫法测定环磷酸鸟苷所必不可少的试剂,它们的放化纯将直接影响这两种分析方法的成败。本工作采用高压反相分配色谱法,在ODS术上,用甲醇-20 mmol/l KH_2PO_4作流动
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| 关 键 词： | 环核苷酸 [~3H]CAMP [~3H]CGMP 高压反相液相色谱 |
ANALYSIS OF [~3H] CAMP AND [~3H] CGMP BY HPLC |
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| WU SHUYUN,MAO QINGNAN,LIN ZHIHAO,LAI YUNXIANG Institute of Atomic Energy,P. O. Box ,Beijing. ANALYSIS OF [~3H] CAMP AND [~3H] CGMP BY HPLC[J]. Atomic Energy Science and Technology, 1987, 0(2) |
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| Authors: | WU SHUYUN MAO QINGNAN LIN ZHIHAO LAI YUNXIANG Institute of Atomic Energy P. O. Box Beijing |
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| Affiliation: | WU SHUYUN,MAO QINGNAN,LIN ZHIHAO,LAI YUNXIANG Institute of Atomic Energy,P. O. Box 275,Beijing |
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| Abstract: | Using high pressure reversed phase liquid chromatography, [~3H] CAMP and[~3H] CGMP were determined successfully. The column was HC-ODS-SIL-Xcolumn, and the moving phase was MeOH-20mmol/l KH_2PO_4 (10/90 or 3/97,V/V, pH=5.5). The analytical time was less than 10min. The measuring precisionwas 1.5% (UV) and 0.15% (radioactivity). The determination limit was 10~(-9)gand 10~2Bq with the detection efficiency of 62%. |
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| Keywords: | Cyclo-nucleotide [~3H] CAMP [~3H] CGMP HPLC |
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