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Growth of Listeria monocytogenes on vacuum-packaged horsemeat for human consumption
Authors:Bonilauri P  Liuzzo G  Merialdi G  Bentley S  Poeta A  Granelli F  Dottori M
Affiliation:

a Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Sezione di Reggio Emilia, Via Pitagora 2, 42100, Reggio Emilia, Italy

b Azienda Sanitaria Locale Modena, Servizio Veterinario Distretto di Sassuolo, Via F. Cavallotti 136, 41049 Sassuolo, Modena, Italy

c Dipartimento di Salute Animale, Sezione di Ispezione degli alimenti di Origine Animale, Università di Parma, Via del Taglio 8, 43100, Parma, Italy

d Azienda Sanitaria Locale Reggio Emilia, Servizio Veterinario Distretto Reggio Nord, Via Circondaria 26, 42015, Correggio, Reggio Emilia, Italy

Abstract:In order to investigate the likelihood of Listeria monocytogenes (serotype 4b, ATCC 19115) growth on vacuum-packaged horsemeat at refrigeration temperature, fourteen horsemeat surface/volume homogeneous 150 g weight pieces were superficially inoculated with serotype 4b L. monocytogenes and vacuum packaged. The samples were stored at 4 ± 1 °C. Two pieces (one for pH determination and one for L. monocytogenes counts) were examined at days 0, 7, 14, 21, 28, 35 and 42. Surface pH did not show significant variations during the experiment. The average L. monocytogenes initial contamination level was 1.77log10 CFU/g. A lag phase of 7 days was recorded. The exponential growth rate between day 7 to day 35 was 0.125log10 CFU/day, corresponding to 3.51log10 CFU/g in 28 days. At the end of the experiment the mean L. monocytogenes log10 CFU/g was 5.78.
Keywords:Listeria monocytogenes  Vacuum packaging  Horsemeat
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