Optimising DNA binding to carbon nanotubes by non-covalent methods |
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| Authors: | Vanesa Sanz Ewa Borowiak Petar Lukanov Anna Marie Galibert Emmanuel Flahaut Helen M Coley S Ravi P Silva Johnjoe McFadden |
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| Affiliation: | aFaculty of Health and Medical Sciences, University of Surrey, Guildford GU2 7XH, United Kingdom;bNano-Electronics Centre, Advanced Technology Institute, University of Surrey, Guildford GU2 7XH, United Kingdom;cSzczecin University of Technology, Centre of Knowledge Based Nanomaterials, Pulaskiego St. 10, 70-322 Szczecin, Poland;dUniversite Paul Sabatier-Inter-University Research and Engineering Centre on Materials (CIRIMAT), 118 Route de Narbonne, F-31062 Toulouse Cedex 9, France |
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| Abstract: | The use of carbon nanotubes as a gene delivery system has been extensively studied in recent years owing to its potential advantages over viral vectors. To achieve this goal, carbon nanotubes have to be functionalized to become compatible with aqueous media and to bind the genetic material. To establish the best conditions for plasmid DNA binding, we compare the dispersion properties of single-, double- and multi-walled carbon nanotubes (SWCNTs, DWCNTs and MWCNTs, respectively) functionalized with a variety of surfactants by non-covalent attachment. The DNA binding properties of the functionalized carbon nanotubes were studied and compared by electrophoresis. Furthermore, a bilayer functionalization method for DNA binding on SWCNTs was developed that utilized RNA-wrapping to solubilize the nanotubes and cationic polymers as a bridge between nanotubes and DNA. |
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