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Improved cellulase production by stable Clostridium cellulolyticum mutants |
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| Authors: | J. Tchunden E. Petitdemange G. Raval H. Petitdemange R. Gay |
| Affiliation: | Laboratoire de Chimie Biologique l, Faculté des Sciences, Université de Nancy l, BP 239, 54506, Vandoeuvre les Nancy, France |
| Abstract: | Methods for the production of mutants of the cellulase producer Clostridium cellulolyticum ATCC 35319 were examined using an agar plate screening technique. Spontaneous and UV light-induced mutants were isolated, some of which exhibited a high level of both endoglucanase and exoglucanase activities as assayed using CMC (carboxymethyl cellulose) and PNPCb (paranitrophenyi-β-cellobioside) respectively. The volumetric enzyme activities were up to 2.5 times those of the wild strain. |
| Keywords: | Clostridium cellulolyticum cellulose cellulase mutants ultra-violet radiation |
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