高功率发光二极管诱导荧光微流控芯片分析小型化检测器的研制

以高功率发光二极管（LED）为激发光源，研制了一种新型的小型化LED诱导荧光检测器，用于微流控芯片分析检测。利用MOS管的压控恒流原理，设计恒流驱动电路，使高功率LED发光稳定。通过设计和制造光学结构，成功将LED的发散光聚焦成约3.5mm×0.3mm的线状光束，可方便地与微流控芯片中的微通道对准，避免了复杂的机械校准结构。光学系统体积大大减少，约为9.5cm×4cm×17cm。用荧光染料NBD和高密度脂蛋白评价该体系的性能，结果表明LED激发光源稳定，检测器重复性好；样品峰形良好，可用于微芯片毛细管电泳分析检测。基本实现了LED诱导荧光检测器的微型化。

A miniaturized high-power light-emitting diode- induced fluorescence detector for microchips analysis

A novel miniaturized fluorescence detector which using a blue high-power light-emitting diode(LED) as an excitation light source for microfluidic chips analysis was developed and evaluated. LED was driven by the current between source and drain, which was invariable when MOSFET was worked in saturated zone. LED generated stable light with the constant current. Special optical structure was designed and manufactured to focalize the divergent light which was emitted by LED into linear beam of light. The size of the beam of light was about 3.5mm×0.3mm. This linear beam of light could easily calibrate itself to microchannel of microfluidic chips. Then it could reduce complexity of detector and miniaturize the detector. And the bulk of the optical system was about 9.5cm×4cm×17cm. NBD C6-ceramide and high density lipoprotein were used to evaluate performance of the detector. The results indicated that LED excitation light source was stable, the detector had good reproducibility and the detector could be used in the microchips capillary electrophoresis analysis. The LED-induced fluorescence detector was miniaturized.