Enhanced regeneration of rice (Oryza sativa L.) embryogenic callus by light irradiation in growth phase

The effect of light irradiation on the growth and regeneration of embryogenic rice calli was investigated. Rice calli grew slightly better in the light than in the dark during the growth stage. When calli subcultured under light irradiation were transferred into a liquid regeneration culture system, the number of plantlets with shoots regenerated from calli subcultured in the light was higher than in the case of those subcultured in the dark, whereas there was no obvious difference between the light and dark subcultures when a solid medium was used for the regeneration. The number of regenerated plantlets increased with increasing light intensity up to 2000 lx, but remained the same when the light intensity was raised further. The maximum number of regenerated plantlets was obtained under a photoperiod of 16 h/d. About 8800 plantlets per liter-medium were regenerated from light-cultured calli, which is 1.5 times more than the number regenerated from calli grown in the conventional dark culture.