Validation of an optical surface plasmon resonance biosensor assay for screening (fluoro)quinolones in egg,fish and poultry |
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Authors: | A.-C. Huet C. Charlier S. Weigel S. Benrejeb Godefroy Ph. Delahaut |
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Affiliation: | 1. Cer Groupe , Santés animale et humaine , Rue Point du Jour 8, 6900 Marloie, Belgium ac.huet@cergroupe.be;3. Cer Groupe , Santés animale et humaine , Rue Point du Jour 8, 6900 Marloie, Belgium;4. Rikilt, Institute of Food Safety , Wageningen UR, P.O. Box 230, 6700 AE Wageningen, The Netherlands;5. Food Research Division , Health Canada , Ottawa, Ontario, Canada K1A 0L2 |
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Abstract: | A surface plasmon resonance biosensor immunoassay has been developed for multi-residue determination of 13 (fluoro)quinolone antibiotics in poultry meat, eggs and fish. The following performance characteristics were determined according to the guidelines laid down for screening assay validation in European Decision 2002/657/EC: detection capability, specificity/selectivity, decision limit, repeatability, ruggedness and stability. The detection capability estimated for norfloxacin, the reference fluoroquinolone, was below 0.5, 1 and 1.5 ng g?1 for poultry meat, egg and fish, respectively. The screening assay proved specific and showed satisfactory sensitivity below the MRL levels even though flumequine and oxolinic acid had lower cross-reactivities. A wide range of non-MRL substances were also detected at concentrations below 10 ng g?1. Repeatability was good with both intra- and inter-assay coefficients of variation <6%; ruggedness was also demonstrated. |
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Keywords: | screening, biosensor veterinary drug residues, fluoroquinolones animal products, meat eggs fish |
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