| ELISA方法检测肝癌血清标志物的应用 |
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| 引用本文： | 王文,王锦红,缪晓辉,Mark A Feitelson,戚中田. ELISA方法检测肝癌血清标志物的应用[J]. 中国生物制品学杂志, 2010, 23(3) |
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| 作者姓名： | 王文 王锦红 缪晓辉 Mark A Feitelson 戚中田 |
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| 作者单位： | 王文,王锦红,戚中田(第二军医大学微生物学教研室,上海市医学生物防护重点实验室,上海,200433);缪晓辉(上海长征医院感染科,上海,200003);Mark A Feitelson(Department of Biology,College of Science and Technology,Temple University(Philadelphia,PA 19122,United States)) |
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| 基金项目： | 上海市学科建设项目，艾滋病和病毒肝炎等重大传染病防治专项 |
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| 摘 要： | 目的对已建立的肝癌血清标志物ELISA检测方法进行扩大规模临床样本检测,进一步评价和优化该方法。方法利用本实验室建立的肝癌血清标志物ELISA检测方法,对407份肝病患者血清和95份正常献血员血清进行检测,并对检测结果进行分析。结果324份HBV感染后的肝癌、肝硬化及肝炎患者血清样本中,2种或2种以上指标阳性比例分别为77.6%、76.1%和52.5%,肝癌患者3种或3种以上指标阳性比例较肝硬化和肝炎患者分别高39.9%和310%;在正常献血员、药物性肝炎、酒精性肝炎及其他类型的癌症患者中,87.5%～100%的患者肝癌血清标志物分子≤1种;URG7是最早出现的抗体阳性标志物分子,URG11和S15a抗体则在进展后期或已发展为肝癌的患者血清中呈优势比例出现。结论5种肝癌血清标志物分子与肝癌发生的风险呈正相关,已建立的肝癌血清标志物ELISA检测方法可用于肝癌高危人群的筛选及小肝癌的早期诊断,作为目前AFP和MRI诊断方法的有益补充。
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| 关 键 词： | 肝癌 血清标志物 ELISA |
Detection of Serum Markers of Hepatocellular Carcinoma by ELISA |
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| Mark A Feitelson. Detection of Serum Markers of Hepatocellular Carcinoma by ELISA[J]. Chinese Journal of Bilogicals, 2010, 23(3) |
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| Authors: | Mark A Feitelson |
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| Abstract: | Objective To further evaluate and optimize the developed ELISA for detection of serum markers of hepatocellular carcinoma(HCC).Methods The serum samples from 407 patients with liver diseases and 95 healthy blood donors were detected by ELISA developed by the authors,and the results were analyzed.Results Of the 324 serum samples from patients with HCC,cirrhosis and hepatitis B,77.6%,76.1%and 52.5%were positive for at least 2 serum markers respectively.The positive rates of at least 3 serum markers in the pat... |
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| Keywords: | ELISA Hepatocellular carcinoma (HCC) Serum marker ELISA |
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