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Mandibular Gland Secretion of Melipona beecheii: Chemistry and Behavior
Authors:Leopoldo?Cruz-López  author-information"  >  author-information__contact u-icon-before"  >  mailto:lcruz@tap-ecosur.edu.mx"   title="  lcruz@tap-ecosur.edu.mx"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author,Edi?A.?Malo,E.?David?Morgan,Manuel?Rincon,Miguel?Guzmán,Julio?C.?Rojas
Affiliation:(1) Departamento de Entomología Tropical, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, Tapachula, Chiapas, CP 30700, AP 36, México;(2) Chemical Ecology Group, Lennard-Jones Laboratory, School of Chemistry and Physics, Keele University, Staffs, ST5 5BG, England;(3) Facultad de Ciencias Químicas, Universidad Autónoma de Chiapas, Carretera Antiguo Aeropuerto Km. 2.0, Tapachula, Chiapas, CP 30700, México
Abstract:The mandibular gland secretion of Melipona beecheii contains a rich mixture of terpenoid and oxygenated compounds and unsaturated and saturated hydrocarbons. However, it differs markedly from the 11 other Brazilian species examined in previous studies, both by the absence of 2-heptanol and the presence of rose oxides. The most abundant compound was geranyl hexanoate, whereas the most volatile compounds were cis- and trans-rose oxide and geraniol. The complete blend and five individual components found in the gland secretion were tested by electroantennography (EAG) and behavioral assays. The complete mandibular gland extract and geraniol elicited the strongest EAG responses, whereas these and farnesyl acetate induced the strongest attack response from workers. The role of the rose oxides remains to be elucidated, as they do not appear to play a major role as an alarm pheromone of this species.
Keywords:Stingless bees  mandibular glands  cis-rose oxide  trans-rose oxide  geraniol  alarm response  electroantennography
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