Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR |
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| Authors: | B Jaulhac M Reyrolle YK Sodahlon S Jarraud M Kubina H Monteil Y Piémont J Etienne |
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| Affiliation: | Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hopitaux Universitaires de Strasbourg, France. jaul-ibs@tpgnet.net |
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| Abstract: | A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate. |
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