| 长江中下游地区6个克氏原螯虾群体遗传多样性分析 |
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| 引用本文： | 黄羽,戴银根,毕成武,胡成钰.长江中下游地区6个克氏原螯虾群体遗传多样性分析[J].南昌大学学报(工科版),2011,33(3):243-247. |
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| 作者姓名： | 黄羽 戴银根 毕成武 胡成钰 |
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| 作者单位： | 1. 南昌大学生命科学与食品工程学院,江西南昌,330031
2. 江西省水产技术推广站,江西南昌,330046 |
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| 基金项目： | 江西省农业厅重点科技攻关资助项目(E04723) |
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| 摘 要： | 为探讨长江中下游地区克氏原螯虾群体遗传多样性，使用AFLP标记，用5个EcoRI—MseI引物从6个群体139个个体中共扩增出129个位点，其中多态性位点为89，占68．99％，通过NTSYSpc软件对统计的I／O矩阵进行分析。结果表明：在群体水平上，洪泽湖显示了最大的遗传多样性，多态位点比例为75．74％，有效等位基因数为1．4181，Nei’S遗传多样性指数为0．5829，Shannon多样性信息指数为0．441；群体问发生了较高程度的遗传分化，6个群体问的遗传分化系数为0．4186，群体的遗传变异91．20％来源于群体内，8．80％来源于群体间，遗传变异主要存在于群体内，群体内的遗传分化大于群体间的分化；6个克氏原螯虾群体可以分为3组，而鄱阳湖群体在这3组中均有分布。
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| 关 键 词： | 克氏原螯虾 AFLP 遗传多样性 长江中下游 |
Assessment of 6 Wild Crayfish for Evaluation of Genetic Diversity in the Middle and Lower Reaches of the Yangtze River |
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| HUANG Yu,DAI Yin-gen,BI Cheng-wu,HU Cheng-yu.Assessment of 6 Wild Crayfish for Evaluation of Genetic Diversity in the Middle and Lower Reaches of the Yangtze River[J].Journal of Nanchang University(Engineering & Technology Edition),2011,33(3):243-247. |
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| Authors: | HUANG Yu DAI Yin-gen BI Cheng-wu HU Cheng-yu |
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| Affiliation: | HUANG Yu1,DAI Yin-gen2,BI Cheng-wu1,HU Cheng-yu1(1.School of Life Science and Food Engineering,Nanchang University,Nanchang 330031,China,2.Fishery Technical Extension Station of Jiangxi Province,Nanchang 330046,China) |
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| Abstract: | The objective of this work is to estimate the genetic diversity using amplified fragment length polymorphism(AFLP).A total of 129 loci were generated with five EcoRI-MseI primer combinations and scored as binary data in 139 individuals.The percentage of polymorphic loci was 68.99%.These data were analyzed by cluster methods in the software package NTSYSpc:Among the six populations,the highest gene diversity was found within the Nanjing(HZ) population: PPB=75.74%,Ne=1.4181,Nei's(1973) gene diversity H=0.5829... |
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| Keywords: | Procambarus clarkii AFLP marker genetic diversity middle and lower reaches of the Yangtze River |
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