β-gliadin proteins from Maris Widgeon wheat

Four β-gliadins have been isolated from the English wheat, Maris Widgeon by Sephadex SP C25 ion-exchange chromatography of the gliadin mixture followed by G-75 or G-100 gel-filtration of either the native or reduced and alkylated proteins. Amino acid analyses of the proteins indicate similar compositions of β₂- and β₄-gliadins and also of β₃- and β₅-gliadins. Minimal molecular weights from amino acid analysis for all four proteins lie within the range 28000 to 30000.