| 森林脑炎纯化疫苗的研究 |
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| 引用本文： | 宋宗明,韩亮,郭新,洪成龙,赵红丽,宋建军,刘双军,钱依群,王全,冉姝,岳立广,惠连,崔文广. 森林脑炎纯化疫苗的研究[J]. 中国生物制品学杂志, 2002, 15(1): 32-34 |
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| 作者姓名： | 宋宗明 韩亮 郭新 洪成龙 赵红丽 宋建军 刘双军 钱依群 王全 冉姝 岳立广 惠连 崔文广 |
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| 作者单位： | 长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所，长春生物制品研究所 长春1300 |
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| 摘 要： | 目的 制备森林脑炎纯化疫苗。方法 将森林脑炎病毒感染地鼠肾单层细胞，收获病毒液，经超滤浓缩、柱层析纯化病毒后配制而成。结果 疫苗兔疫保护力指数均≥1 .0 ×106，杂蛋白去除率≥99％，小牛血清蛋白去除率＞99％，所有检测项目全部合格。结论 该纯化工艺制备的森林脑炎纯化疫苗抗原含量高，热稳定性好，免疫原性强，各项检测结果均符合2000年版《中国生物制品规程》标准。
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| 关 键 词： | 森林脑炎病毒 柱层析 纯化疫苗 |
| 修稿时间： | 2001-09-06 |
Preparation of Purified Tick-borne Encephalitis Vaccine |
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| SONG Zongming,HAN Liang,GUO Xin et al. Preparation of Purified Tick-borne Encephalitis Vaccine[J]. Chinese Journal of Bilogicals, 2002, 15(1): 32-34 |
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| Authors: | SONG Zongming HAN Liang GUO Xin et al |
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| Abstract: | [ Abstract] Objective To prepare purified tick - borne encephalitis vaccine. Methods Hamster kidney cells were infected with tick - borne encephalitis virus,and the harvested virus suspension was concentrated by ultrafiltration and purified by column chromatography. Results The protection index of the prepared vaccine was not less than 1.0 ×106, and at least 99% of foreign and bovine serum proteins in it were removed. All the indexes of it met the Chinese Requirements for Biologies, 2000. Conclusion The purified tick - borne encephalitis vaccine showed high antigen content,good thermal stability and strong immunity. |
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| Keywords: | Tick - borne encephalitis Column chromatography Purified vaccine |
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