cis-acting elements that mediate the negative regulation of Moloney murine leukemia virus in mouse early embryos |
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| Authors: | M Vernet J Cebrian |
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| Affiliation: | Institut Cochin de Génétique Molécularire, Laboratoire de Génétique et Pathologie Expérimentales, Unité 380 de l'Institut National de la Santé et de la Recherche Médicale, Paris, France. |
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| Abstract: | We have addressed the question of the nature of Moloney murine leukemia virus (MoMuLV) repression in mouse embryos by assaying for the transient expression of MoMuLV-derived constructs microinjected into early cleavage embryos. We show that the same cis-acting DNA sequences responsible for the block in MoMuLV expression in embryonal carcinoma cell lines operate in early embryos: (i) the MoMuLV long terminal repeat is nonfunctional, and (ii) the +147 to +163 repressor binding site, or negative regulatory element, negatively regulates the expression from an active promoter. |
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