| Abstract: | Synthesis of protein and amino acids by the thermotolerant yeast Candida tropicalis K-41 was studied at various temperatures of cultivation during growth on media with hydrocarbons in conditions of continuous cultivation. The content of protein in the cells decreased by 6% with an increase in growth temperature by 10 degrees C (from 29 to 39 degrees C) while excretion of free amino acids into the medium increased. The cultural broth of the cells grown at 39 degrees C contained up to 8 amino acids, with alanine, aspartic acid, serine and glycine prevailing. The content of amino acids which liberated into the medium decreased in the intracellular pool. However, the total amount of free amino acids in the system "cell--medium" remained constant at various temperatures of cultivation. Synthesis of free amino acids does not presumably limit total synthesis of protein by the yeast. |
