HIV-1中国流行株gp120基因在痘苗病毒中的表达及其与核酸疫苗的实验免疫研究

将HIV－1中国流行株gp120基因在痘苗病毒中进行表达，以期获得重组痘苗病毒，与核酸疫苗混合免疫，评价免疫效果，为艾滋病疫苗开发研制打下基础。将HIV－1中国流行株gp120基因片段插入到pJ38载体启动子下游，经同源重组和血凝素阴性空斑筛选重组痘苗病毒，SDS－PAGE、Western blot检测目的蛋白。以重组病毒和核酸疫苗免疫BALB／c小鼠，进行淋巴细胞转化实验、CTL、CD4＋CD8＋T细胞数目以及血清抗体等细胞免疫和体液免疫指标检测。结果获得的重组痘苗病毒vJ38pg120能够表达Gp120蛋白并诱导机体产生细胞免疫和体液免疫，具有良好的免疫原性，其免疫效果以2rVV-DNA混合方式为最好。重组痘苗病毒vJ38gp120。

Study on Construction and Experimental Immunity of Recombinant Vaccinia Viruses and Nucleic Acid Vaccine Plasmids of HIV-1 gp120

To obtain recombinant vaccinia viruses of gp120 gene of HIV 1 subtype B and evaluate the immune effects after immunization with nucleic acid vaccine for AIDS vaccine development. gp120 gene of HIV 1 subtype B was inserted downstream of the combined promotor of pJ38 vector. Recombinant vaccinia viruses were selected by using plaque assay after homologous recombination. Expression products were examined by SDS PAGE and Western blot. Immune response of BALB/c mice was evaluated by lymphocyte transformation test, CTL, quantity change of T lymphocyte of CD + 4, CD + 4and OD value of serum IgG antibody. Results showed that recombinant vaccinia viruses vJ38gp120 were obtained with reactinogenicity. Effect of rVV immunized is very good, the best one is the rVV primed and nucleic acid vaccine boosted group. Gp120 protein can be expressed in vJ38gp120 and cellular immunity and humoral immunity of organism were induced.