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纳豆咀嚼片中总异黄酮的含量测定
引用本文:蒋志勤.纳豆咀嚼片中总异黄酮的含量测定[J].食品研究与开发,2009,30(7).
作者姓名:蒋志勤
作者单位:天津师范大学,化学与生命科学学院,天津,300074
摘    要:建立测定纳豆咀嚼片中总异黄酮含量的方法-紫外分光光度计法,以染料木素作为对照品,利用染料木素与氢氧化钠产生反应,并在275 nm波长处产生最大吸收峰的特性,用紫外分光光度法测定纳豆咀嚼片中总异黄酮含量.测定结果:纳豆咀嚼片中总异黄酮的含量为325μg/片,加样回收率为98.3%.

关 键 词:纳豆咀嚼片  异黄酮  紫外光谱法

QUANTITATIVE DETERMINATION OF TOTAL ISOFLAVORNES IN NATTO CHEWABLE TABLETS BY ULTRA-VIOLET SPECTROPHOTOMETRY
JIANG Zhi-qin.QUANTITATIVE DETERMINATION OF TOTAL ISOFLAVORNES IN NATTO CHEWABLE TABLETS BY ULTRA-VIOLET SPECTROPHOTOMETRY[J].Food Research and Developent,2009,30(7).
Authors:JIANG Zhi-qin
Abstract:Establish a UV spectrophotometry for determination of isoflavones in Natto Chewable Tablets,Genistein was selected as the reference substance in this investigation. Based on the reaction of genistein and NaOH, which has the maxium absorption in 275 nm wavelength, spectrophotometry method was used for determination of isoflavones in Natto Chewable Tablets, the total content of isoflavones in Natto Chewable Tablets extracts was 325 μg/tablet with mean recovery 98.3%.
Keywords:Natto chewable tablets  isoflavone  Ultra-Violet spectrophotometry
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