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Lethal effect of the expression of a killer gene SMK1 in Saccharomyces cerevisiae |
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| Authors: | Suzuki C; Kawano M; Kashiwagi T; Arata Y; Kawasumi T; Kashiwagi Y |
| Affiliation: | National Food Research Institute, 2-1-2 Kannondai, Tsukuba, Ibaraki305-8642, 2 Japan Women's University, Faculty of Home Economics,2-8-1 Mejirodai, Bunkyo-ku, Tokyo 112-8681, 3 Protein Engineering Research Institute, 6-2-3, Furuedai, Suita, Osaka 565-0874 and 5 Water Research Institute, 2-1-6, Sengen, Tsukuba, Ibaraki 305-0047, Japan |
| Abstract: | Expression of the SMK1 gene which encodes the yeast killer toxinSMKT is lethal in Saccharomyces cerevisiae. Effects of deletionand site-directed mutagenesis of SMK1 on the lethality and thesecretion of the gene products were examined. Deletion of theinterstitial |
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