Nitric oxide-scavenging and antioxidant effects of Uraria crinita root |
| |
| Affiliation: | 1. Bio-Nano Laboratory, PG and Research Department of Physics, Arul Anandar College, Karumathur – 625514, India;2. MOE Key Laboratory for Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou-310027, PR China;3. School of Physics, Madurai Kamaraj University, Madurai – 625 024, India;4. Department of Materials Science and Nano-engineering, Rice University, Texas 77005 189, United States;1. Hebei Key Lab of Optic-electronic Information and Materials, College of Physics Science and Technology, Hebei University, Baoding 071002, PR China;2. College of Chemistry and Material Science, Key Laboratory of Inorganic Nano-materials of Hebei Province, Hebei Normal University, No. 20 Rd. East of 2nd Ring South, Yuhua District, Shijiazhuang City, Hebei Province, 050024, PR China;1. Institute of Macromolecular Chemistry, AS CR, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic;2. Palladin Institute of Biochemistry, NASU, 9 Leontovich St., 01601 Kiev, Ukraine;3. R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, 45 Vasylkivska St., 03022 Kiev, Ukraine;1. 129 Machon Bank Road, Sheffield, UK;2. Delta Hat Limited, 1 Lamorna Court 43 Wollaton Road Beeston, Nottingham, UK;3. Department of Statistical Science, University College London Standard Institution, London, UK;1. Department of Applied Informatics, University of Szeged, Boldogasszony sgt. 6, H-6725 Szeged, Hungary;2. Central Clinical Pharmacy, University of Debrecen, Nagyerdei krt 98, H-4032 Debrecen, Hungary;3. Department of Pharmaceutical Technology, University of Szeged, Eötvös u. 6, H-6720 Szeged, Hungary;1. Department of Chemistry and Chemical Engineering, Jiangxi Normal University, 330022, Nanchang, China;2. Institute of Urban Environment Chinese Academy of Sciences, 1799 Jimei Road, 361021, Xiamen, China |
| |
| Abstract: | The antioxidant activity and the nitric oxide-scavenging effect of methanol extracts (MEUR), and their ethyl acetate fraction (EAF), from Uraria crinita root, were investigated. The results of the comet assay indicated that both MEUR and EAF could inhibit DNA damage in macrophage induced by sodium nitroprusside at a concentration of 25–200 μg/ml. The antioxidant activity of the extracts was determined using the Trolox equivalent antioxidant capacity (TEAC) assay. The TEAC values of MEUR and EAF were 0.2 and 0.4, respectively, at a concentration of 200 μg/ml. The antioxidant activity and nitric oxide-scavenging effect of MEUR and EAF showed concentration-dependence. In addition, the results also showed a decreasing effect on nitric oxide production of lipopolysaccharide-induced RAW 264.7, for both extracts. One of the major antioxidative components was isolated from EAF and was identified as genistein, on the basis of UV-vis spectral, IR, MS and NMR analyses. Thus, the traditional edible plant, Uraria crinita, may have potential as an antioxidant and as a nitric oxide-scavenging agent. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
