Optimization of acid hydrolysis conditions for total isoflavones analysis in soybean hypocotyls by using RSM |
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| Affiliation: | 1. Department of Chemistry, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan;2. Department of Chemistry, Federal Urdu University of Arts, Science and Technology, Gulshan-Iqbal, Karachi, Pakistan;3. National Centre of Excellence in Analytical Chemistry University of Sindh, Jamshoro, Pakistan;4. Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, 155 Yang Qiao West Road Fuzhou, Fujian 350002, China;1. Department of Chemistry, Art and Science Faculty, Balikesir University, 10100 Balikesir, Turkey;2. School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK;1. Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing 100875, China;2. Analytical and Testing Center, Beijing Normal University, China |
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| Abstract: | The optimum hydrolysis conditions of soybean hypocotyls with HCl for a quantitative HPLC determination of total isoflavones (TI) were studied by using response surface methodology (RSM). HCl concentration (HC), hydrolysis time (HT) and reaction temperature (RT) were assumed to be the most important factors affecting hydrolysis for the determination of TI. Optimum hydrolysis conditions for maximizing the determination of TI were: HC=3.42N, HT=205.5 min and RT=44.6°C. The model had a satisfactory coefficient of R2 (=0.967) and was verified experimentally. Furthermore, the recovery of individual isoflavones was 97–100% under the optimum hydrolysis conditions. The results suggested that the conditions were mild and useful for maximizing a quantitative HPLC determination of TI in soybean hypocotyls. |
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