Factors influencing ultraviolet and electron beam irradiation-induced free radical damage of ascorbic acid |
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| Affiliation: | 1. Chemical and Biological Engineering Department, Center for Micro-Engineered Materials (CMEM), University of New Mexico, Advanced Materials Lab, 1001 University Blvd. SE Suite 103, MSC 04 2790, Albuquerque, NM 87131. United States;2. CFD Research Corporation, 701 McMillian Way Northwest, Huntsville, AL 35806, United States;1. State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China;2. Ecological Technique and Engineering College, Shanghai Institute of Technology, Shanghai, 201418, PR China;1. Department of Macromolecular Physics, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 180 00 Prague 8, Czech Republic;2. Centro de Biomateriales e Ingeniería Tisular, Universitat Politècnica de València, Camino de Vera s/n, E-46022 Valencia, Spain;3. Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Zaragoza, Spain;4. University of West Bohemia, New Technologies Research Centre, Univerzitní 8, 30614 Plzeň, Czech Republic |
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| Abstract: | The oxidation of an unsaturated fatty acid and antioxidant vitamin by ultraviolet (UV) and electron beam (e− beam) irradiation was studied. Hydroxyl radical ( OH), generated by Fe-EDTA and hemoglobin, under heat incubation or UV illumination, was compared. L-ascorbic acid (LAA; 10, 100 and 500 mM) oxidation was measured after irradiation at 0–10 kGy, in the presence and absence of myoglobin. Moreover, both UV and e− beam irradiation at 0–20 kGy were used to oxidize a linoleic acid emulsion, both with and without LAA. The presence of hemoglobin generated less OH, than Fe-EDTA under similar heating conditions. Generation of OH increased with illumination by UV. LAA was oxidized by e− beam irradiation, with an increasing rate of oxidation occurring at lower concentrations of LAA and higher dosages of irradiation. LAA (10 mM) exhibited pro-oxidant activity in the linoleic acid emulsion when exposed to both forms of irradiation, while 500 mM LAA exhibited antioxidant activity. |
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