| 高效液相色谱法测定异麦芽低聚糖的组分 |
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| 引用本文： | 王清,郭家荣.高效液相色谱法测定异麦芽低聚糖的组分[J].食品与发酵工业,1998,24(4):28-30. |
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| 作者姓名： | 王清 郭家荣 |
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| 作者单位： | 中国食品发酵工业研究所 |
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| 摘 要： | 采用高效液相色谱法，以Ｓｐｈｅｒｉｓｏｒｂ－ＮＨ２色谱柱和ＷａｔｅｒｓＲ４０１示差折光检测器对异麦芽低聚糖中各组分进行快速地分离测定。分析结果表明，分离效果良好，样品中异麦芽低聚糖含量达６０％以上。
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| 关 键 词： | 高效液相色谱法 异麦芽低聚糖 |
Determination of Composition of Isomaltooligosaccharide by High Performance Liquid Chromatography |
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| Wang Qing,Guo Jiarong,Zhang Lu.Determination of Composition of Isomaltooligosaccharide by High Performance Liquid Chromatography[J].Food and Fermentation Industries,1998,24(4):28-30. |
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| Authors: | Wang Qing Guo Jiarong Zhang Lu |
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| Abstract: | Isomaltooligosaccharide is produced from starch by enzymatic coversion,transglycosylation and purification. Isomaltooligosaccharide is used as less sweet taste and low calorie sweetener. It is effective for the growth of Bifidobacteria and beneficial in preventing dental caries. It also enhances immunity of human body. This method is used to analyze the sugar composition by HPLC with column of Spherisorb NH 2 300mm×5mm i.d. and Waters R401 differential refractometer. The results indicate that separation effect is satisfactory.The sample prepared in our laboratory contains above 60% isomaltooligosaccharide. This method is simple,rapid,accurate and suitable for analysing the composition of isomaltooligosaccharide in study and production. |
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| Keywords: | HPLC isomaltooligosaccharide |
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