多重PCR合成天蚕素基因及其在E.Coli中的克隆

天蚕素抗菌肤是食品领域中研究最多的昆虫抗菌多肤。通过对62种天蚕素低级结构的研究，设计出4条全新抗菌肤序列。应用多重PCR方法合成设计多肤的全基因序列，酶切PCR扩增产物和克隆载体puc19，构建重组质粒并将其转化到大肠杆菌中。测序结果表明目的片段已经成功克隆到宿主菌JM109中。

Synthesis and Clone of Cecropins' Gene in E.coli

Cecropins is a group of insect antibacterial peptide, which is generally researched in food science.By utilizing bioinformatics we studied sixty-two cecropins and designed four cecropins-like polypeptides.The four polypeptides were reverse-translated into 202bp gene based on Escherichia coli bias codons.Objective: synthesizing and cloning the antibacterial peptides' gene.Methods: utilizing multiple PCR,we synthesized total genes of design polypeptides,constructed recombinant plasmid and transformed to E.coli.Result: genes of four design polypeptides had been successfully cloned into PUC19 through the verification of both agarose gel electrophoresis and genetic sequencing for recombinant vector.