| SYNTHESIS AND PURIFICATION OF MODIFIED ANTISENSE OLIGODEOXYRIBONUCLEOTIDES |
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| 引用本文： | Li Xiaoru(Department of Chemistry,Central South University of Technology,Changsha 410083,China)Wen Zhibin(Department of Basic Medicine,Hunan Medical University,Changsha 410078,China). SYNTHESIS AND PURIFICATION OF MODIFIED ANTISENSE OLIGODEOXYRIBONUCLEOTIDES[J]. 中南工业大学学报(英文版), 1997, 4(1): 49-53. DOI: 10.1007/s11771-997-0030-3 |
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| 作者姓名： | Li Xiaoru(Department of Chemistry Central South University of Technology Changsha 410083 China)Wen Zhibin(Department of Basic Medicine Hunan Medical University Changsha 410078 China) |
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| 作者单位： | Li Xiaoru(Department of Chemistry，Central South University of Technology，Changsha 410083，China)Wen Zhibin(Department of Basic Medicine，Hunan Medical University，Changsha 410078，China) |
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| 摘 要： | ChemicalsynthesisofnucleicacidshasbeencarriedgreatlyforwardbecauseoftheadventofsolidphasesynthesisandthewideuseofDNAsynthesizerintheearlyeighties,andhasalsobeenledtotheveryattractiveprospectsbytherecentlydevelopedantisensetechnology['].Inabroadsense,antisensetechnologyisanewkindofapproachtoinhibitorsuppressthegeneexpressionbyartificialsynthesizedorbiosynthesizedoligonucleotidefragments(oritschemicalmodifiedcounterparts)whicharespecificcomplementarytoitssensestrand.Alargenumberofreferenceshave…
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| 收稿时间： | 1996-01-03 |
SYNTHESIS AND PURIFICATION OF MODIFIED ANTISENSE OLIGODEOXYRIBONUCLEOTIDES |
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| Li Xiaoru. SYNTHESIS AND PURIFICATION OF MODIFIED ANTISENSE OLIGODEOXYRIBONUCLEOTIDES[J]. Journal of Central South University of Technology, 1997, 4(1): 49-53. DOI: 10.1007/s11771-997-0030-3 |
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| Authors: | Li Xiaoru |
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| Affiliation: | (1) Department of Chemistry, Central South University of Technology, 410083 Changsha, China;(2) Department of Basic Medicine, Hunan Medical University, 410078 Changsha, China |
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| Abstract: | The synthesis and purification of modified antisense oligodeoxyribonucleotides (MAO) are desoribed. A new oligonucleotide purification method and a novel activating agent, dimethyl sulfoxide, for the condensation reaction are used. EDTA has been attached covalently to the 5′ end of the oligodeoxyribonucleotides sequence via an ethylenediamine linker in basic triethylammonium solution, then chelated with the metal ions such as Fe(II), Co(II) or Cu(II). High performance liquid chromatorgaphy is used to separate and purify this kind of MAO, using double mobile phase, triethylammonium acetate and acetonitrile. By using this routine chemical synthesis method, EDTA can be linked to the 5′ terminus of oligodeoxyribonucleotedes of any desired length and sequence, forming chimeric MAOs. Project supported by the China Medical Board of America Synopsis of the first author Li Xiaoru, associate professor, born in April 1963, received Ph D degree in 1993. Specializing in molecular biology and bioorganic chemistry. Research interests are directed at the expression and regulation of oncogenes and antioncogenes, and at gene transfer. |
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| Keywords: | oligodeoxyribonucleotedes synthesis purification high performance liquid chromatography |
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