均匀设计法优化超声波辅助提取甘草总黄酮

目的:优化甘草中总黄酮的提取工艺。方法:在单因素实验基础上,采用均匀设计Ug*(85)表设计方法研究甲醇体积分数、料液比、超声时间对总黄酮提取率的影响,并使用Uniform Design 3.0软件建立甘草总黄酮提取的数学模型,并得最佳工艺条件。结果:甘草总黄酮提取的最佳工艺条件为:甲醇浓度为70%、超声时间为30 min/h、固液比为1∶20,甘草总黄酮的最大得率为3.69%。结论:用均匀设计Ug*(85)法得到的模型方程,可在设立的因素水平范围内给出甘草总黄酮得率极大值,进而探讨各因素对甘草总黄酮得率的影响机制。

Optimization for Ultrasound Assisted Extraction Condition of Total Flavone from Licorice by Uniform Experiment Design

Objective] To optimize the extraction conditions of total flavone from Liquorice. Method] Based on single-factor experiments, the effects of methyl concentration, solid-liquid ratio and extraction time on yield of total fiavone were studied by U8＊（85）, and mathematic model for the extraction conditions of total flavone was established and The optimum extraction conditions were got by the software Unilbrm Design 3.0. Results] The optimum extraction conditions were as following; the methyl concentration 70 %, extraction time 34.84 min, solid-liquid ratio 1 i 20. Under the conditions, yield of total flavone was 3.69 %. Conclusion] By using Ue＊（85）, the optimal yield of total flavone can be calculated by the mathematic model within experiment range, and then the factors affecting the extraction of total flavone can also be investigated.