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PEGylated antibody in organic media |
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| Authors: | Liu Mingzhe Xu Muye Loh Xian Jun Abe Hiroshi Tsumuraya Takeshi Fujii Ikuo Li Jun Son Tae Il Ito Yoshihiro |
| Affiliation: | 1 Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan,;2 Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-1, Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan,;3 Division of Bioengineering, Faculty of Engineering, National University of Singapore, Block EA, #03-12, 9 Engineering Drive 1, Singapore 117576, Singapore,;4 Department of Bioscience & Biotechnology, Chung-Ang University, 40-1 San, Nae-Ri, Daeduck-myun, Ansung-si, Kyungki-do, 456-756, Korea |
| Abstract: | Antibodies were covalently conjugated with poly(ethylene glycol) (PEG) and the properties of the PEGylated antibodies in organic media were investigated. Two types of monoclonal antibody were used in this study. One was a monoclonal antibody (abzyme) that was prepared against a hapten mimicking a transition state of hydrolysis. Another was a monoclonal antibody against estrogen, which is not soluble in water. By electrophoresis and mass spectral analysis, the covalent conjugation with PEG chains was confirmed. The PEGylated antibodies bound to antigens and the PEGylated abzyme catalyzed a hydrolysis reaction in an aqueous solution. The PEGylated antibodies were soluble in dichloromethane and acetone and interacted with antigen either in dichloromethane or in acetone. In conclusion, PEGylated antibodies can be employed as analytical tools for water-insoluble analytes. |
| Keywords: | Antibody Poly(ethylene glycol) Catalytic antibody Organic solvents Immunoassay |
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