Synthesis of silicon-substituted hydroxyapatite by a hydrothermal method with two different phosphorous sources |
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Authors: | Alieh Aminian Mehran Solati-HashjinAli Samadikuchaksaraei Farhad BakhshiFazel Gorjipour Arghavan FarzadiFattolah Moztarzadeh Martin Schmücker |
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Affiliation: | a Nanobiomaterials Lab, Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran 15875-4413, Islamic Republic of Iran b Department of Biotechnology, Cellular & Molecular Research Center, Iran University of Medical Science, Tehran 14155-6183, Islamic Republic of Iran c Bioceramics Lab, Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran 15875-4413, Islamic Republic of Iran d German Aerospace Center (DLR), Institute of Materials Research, Köln, Germany |
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Abstract: | Silicon-substituted hydroxyapatite (Si-HA) with up to 1.8 wt% Si content was prepared successfully by a hydrothermal method, using Ca(NO3)2, (NH4)3PO4 or (NH4)2HPO4 and Si(OCH2CH3)4 (TEOS) as starting materials. Silicon has been incorporated in hydroxyapatite (HA) lattice by partially replacing phosphate (PO43−) groups with silicate (SiO44−) groups resulting in Si-HA described as Ca10(PO4)6−x(SiO4)x(OH)2−x. X-ray diffraction (XRD), Fourier transform IR spectroscopy (FTIR), inductively coupled plasma AES (ICP-AES) and scanning electron microscopy (SEM) techniques reveal that the substitution of phosphate groups by silicate groups causes some OH− loss to maintain the charge balance and changes the lattice parameters of HA. The crystal shape of Si-HA has not altered compared to silicon-free reference hydroxyapatite but Si-incorporation reduces the size of Si-HA crystallites. Based on in vitro tests, soaking the specimens in simulated body fluid (SBF), and MTT assays by human osteoblast-like cells, Si-substituted hydroxyapatite is more bioactive than pure hydroxyapatite. |
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Keywords: | Bioceramics Hydrothermal synthesis Silicon-substituted hydroxyapatite Bioactivity |
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