Multitrack Staining Apparatus with High Resolution and Time-Lapsed Acquisition for Polyacrylamide Gels |
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| Authors: | Praveen Maurye Arpita Basu Jayanta Kumar Biswas |
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| Affiliation: | 1. Fishery Resource and Environment Management Division, Central Inland Fisheries Research Institute (I.C.A.R.), Kolkata, West Bengal, Indiamaurye_p@yahoo.com;3. Fishery Resource and Environment Management Division, Central Inland Fisheries Research Institute (I.C.A.R.), Kolkata, West Bengal, India;4. International Centre for Ecological Engineering, University of Kalyani, Kalyani, West Bengal, India |
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| Abstract: | Polyacrylamide gel electrophoresis (PAGE) is commonly used for the separation, identification, and purification of mixtures of proteins and nucleic acids. A suitable staining method for the polyacrylamide gels is imperative to determine low concentrations of resolved macromolecules. Several staining methods have been adopted; ethidium bromide, silver, and coomassie staining are the most frequently employed. Silver staining determines macromolecules at nanomolar concentrations but involves many steps and thus is tedious for multiple gels in parallel. In this article, a simple multitrack laboratory-constructed silver staining apparatus is characterized that may enhance the sensitivity of the electrophoresis stains and the homogeneity of detection. In addition, the low cost and simplicity of this system provide wide applicability for gel electrophoresis. |
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| Keywords: | polyacrylamide gel proteins nucleic acids silver staining |
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