Sequential Injection Analysis utilizing Amperometric Biosensors as Detectors for the Simultaneous Determination of L- and D-Pipecolic Acid |
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| Authors: | Raluca-Ioana Stefan-van Staden R'afat Mahmoud Nejem Jacobus Frederick van Staden |
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| Affiliation: | 1. Laboratory of Electrochemistry Bucharest , National Institute of Research for Electrochemistry and Condensed Matter , Bucharest, Romania;2. Department of Chemistry , Al-Aqsa University , Gaza, Palestine |
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| Abstract: | Abstract Enantioanalysis of biomedical substances is very important for clinical analysis because the L- and D-enantiomers of the same chiral compound may be markers for different illnesses. A sequential injection analysis (SIA) system using amperometric biosensors as detectors is proposed for the simultaneous determination of L- and D-pipecolic acid (PA). The amperometric biosensors were based on L-amino acid oxidase (L-AAOD) or/and horseradish peroxidase (HRP) for the assay of L-PA and D-amino acid oxidase (D-AAOD) and/or HRP for the assay of D-PA. The linear concentration ranges are of pmol/L to nmol/L magnitude order, with very low limits of detection. The SIA/biosensors system can be used reliably on-line for the simultaneous assay of L- and D-PA from serum samples, with a frequency of 34 samples per hour. |
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| Keywords: | Enantioanalysis L(D)-Pipecolic acid Sequential injection analysis |
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