Automatic Absorbance Calibration Routine for a Computerized Uv-Visible Rapid Scanning Spectrophotometer |
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| Authors: | Alexander M Yacynych Harry B Mark Jr |
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| Affiliation: | 1. Research Institute of Innovative Products and Technologies, The Hong Kong Polytechnic University , Hung Hom , Hong Kong SAR rikflei@polyu.edu.hk;3. Research Institute of Innovative Products and Technologies, The Hong Kong Polytechnic University , Hung Hom , Hong Kong SAR |
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| Abstract: | Sir: A Fortran IV computer program is used to calculate absorbance at varying wavelengths in a UV-visible rapid scanning spectrophotometer. Using the program eliminates the necessity of calibrating the absorbance axis in the usual manner using standard solutions or filters and plotting calibration curves, because the voltage output of the computer is automatically identical to the actual absorbance of a sample. |
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| Keywords: | clinical diagnostics gold nanoparticles microfluidics sandwich immunoassay |
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