HPLC Determination of Sugars, Starch and Oligosaccharides in Infant Formulas using Resin-Based, Fixed-Ion Columns |
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| Authors: | FRASER W. SCOTT GEORGE HATINA |
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| Affiliation: | The authors are with the Nutrition Research Division, Food Directorate, Health Protection Branch, Health &Welfare Canada, Banting Research Centre, Tunney's Pasture, Ottawa, Ontario, Canada, K1A 0L2. |
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| Abstract: | A more detailed pattern of the available carbohydrates in infant formulas, i.e., sugars, starch and total oligosaccharides (measured as oligosaccharide-derived glucose) was obtained using ion-moderated partition chromatography with fixed-ion, resin-based HPLC columns. Twenty-five infant formula products were analyzed. Lactose, the main sugar in standard infant formula, was often associated with its non-absorbed isomer, lactulose. Soy-based formulas contained sucrose and many had substantial amounts of oligosaccharide-derived glucose. Formulas for premature infants had less than half the lactose of standard formulas with oligosaccharide-derived glucose supplying 35 to 57% total carbohydrate. Protein hydrolysate formulas had the highest amounts of starch. |
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