三波长紫外分光光度法测定红车轴草异黄酮含量的研究

红车轴草乙醇提取液，颜色较深，背景吸收较大，以一般常用的单波长紫外分光光度法测定红车轴草异黄酮含量时，色素和醇溶性蛋白等杂质干扰严重。本文建立的三波长紫外分光光度法，能有效扣除色素和醇溶性蛋白等杂质的干扰，精密度实验的相对标准偏差为0．85％，平均加样回收率为103．95％，加样回收率的相对标准偏差为1．82％。本方法具有简便、快速、准确等特点。

Study on Determination of Trifolium pratense Isoflavones by Three-wavelength UV Spectrophotometry

Three-wavelength UV spectrophotometry was established to determine the content of total isoflavones in Trifolium pratense. This method could eliminate interference of pigments and ethanol-soluble proteins in the ethanol extract of Trifolium pratense. The relative standard deviation (RSD) of precision test was 0.85%, the average recovery of adding sample test was 103. 95% and RSD was 1.82%. It was a simple, fast and accurate method for determination of Trifolium pratense isoflavones.