Facile synthesis,in vitro biocompatibility,and antibacterial activity of porous hollow hydroxyapatite microspheres |
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| Authors: | Shuaishuai Li Ren Guo Song Chen Jianan Zhang Toshiyuki Ikoma Xiaona Li Weiyi Chen |
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| Affiliation: | 1. College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, China;2. Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, Tokyo, Japan |
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| Abstract: | In this study, porous hollow hydroxyapatite (HAp) microspheres are prepared using chitosan microspheres as novel sacrificial templates and their microstructure, biocompatibility, and drug delivery properties are evaluated. Scanning electron microscope (SEM) observations show that HAp microspheres are spherical in morphology with a diameter of 100–300 μm and have a porous and core–shell structure. X-ray diffractometer patterns show that HAp microspheres consist of apatite phase. MTT assay indicates that HAp microspheres are biocompatible and have no significant cytotoxicity. SEM observations show that HAp microspheres support attachment and proliferation of osteoblast MC3T3-E1 cells. After being soaked in the solution of tetracycline hydrochloride (TH, model drug), HAp microspheres adsorb TH with an adsorption capacity of 47% to derive TH-loaded HAp microspheres. When exposed to two types of representative bacteria: Escherichia coli and Staphylococcus aureus, TH-loaded HAp microspheres maintain the biological activity of TH to inhibit the growth of bacteria. |
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| Keywords: | bioceramics hydroxyapatite microstructure |
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