| 产单宁酶菌株的筛选及选育 |
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| 引用本文： | 陶素芬,赵祥颖,刘建军.产单宁酶菌株的筛选及选育[J].食品科学,2012,33(1):226-229. |
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| 作者姓名： | 陶素芬 赵祥颖 刘建军 |
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| 作者单位： | 1.山东轻工业学院食品与生物工程学院
2.山东省食品发酵工程重点实验室 |
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| 摘 要： | 为得到高产单宁酶的菌株，从多年种植的茶园、柿子园以及掩埋柿子、五倍子和茶叶的花园土壤中通过平板初筛和三角瓶固体发酵复筛筛选到一株产单宁酶的曲霉菌株DNM1-39，固体培养其产单宁酶活力为1.58U/g。以DNM1-39为出发菌株，经紫外线照射、硫酸二乙酯单独处理和紫外线照射-硫酸二乙酯复合诱变处理，获得突变株UD-6，其产酶活力达2.80U/g。连续传代实验结果表明，该菌株产酶活力不变，性能稳定。
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| 关 键 词： | 单宁酶 单宁 没食子酸 筛选 诱变 |
| 收稿时间： | 2011-01-27 |
Breeding and Screening of a High-Yield Tannase-Producing Strain |
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| TAO Su-fen,ZHAO Xiang-ying,LIU Jian-jun.Breeding and Screening of a High-Yield Tannase-Producing Strain[J].Food Science,2012,33(1):226-229. |
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| Authors: | TAO Su-fen ZHAO Xiang-ying LIU Jian-jun |
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| Affiliation: | 1,2, (1.School of Food and Biological Engineering,Shandong Polytechnic University,Jinan 250353,China; 2.Research and Design Institute of Food and Fermentation Industries of Shandong Province,Jinan 250013,China) |
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| Abstract: | Tannase plays an important role in the food and fine chemical industries.The breeding and screening of a strain with high tannase activity are desired.A tannase-producing strain,DNM1-39,was isolated from resources rich in tannin.The activity of tannase in solid state medium after fermentation by the strain was 1.58 U/g.The strain was identified as Aspergillus through morphologic observation.A mutant strain named as UD-6 was obtained from DNM1-39 mutagenization with ultraviolet irradiation alone,DES treatment alone and both of them.The tannase activity produced by UD-6 was 2.80 U/g.The tannase-producing ability of UD-6 remained the same after continuous passages and displayed excellent genetic stability. |
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| Keywords: | tannase tannin gallic acid screening mutagenesis |
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