百合多糖的药理作用研究进展

龙牙百合（Lilium brownii var. viridulum）是我国主要传统食用药用百合之一，本试验拟探究龙牙百合多糖提取工艺及其理化性质和功能活性，以龙牙百合鳞茎为原料，以多糖得率为指标，料液比、浸提时间和浸提温度为因素，对龙牙百合多糖提取工艺进行优化，再进一步去蛋白纯化多糖，分析其理化性质，测定其体外抗氧化和降血脂能力。结果显示：龙牙百合粗多糖最佳提取工艺为料液比1:20 g/mL，浸提时间20 min，浸提温度75 ℃，粗多糖得率为11.98%。粗多糖总糖、糖醛酸和蛋白质含量分别为58.46%、8.06%和10.85%，经过去蛋白后多糖的总糖和糖醛酸含量分别提高到84.78%和15.41%，蛋白质含量降低至4.73%。龙牙百合去蛋白多糖在10 mg/mL时对DPPH和ABTS⁺自由基清除率为8.75%和38.59%，低于对照V_C；对胰脂肪酶的抑制率为85.78%，高于对照奥利司他，有较好的体外抑制胰脂肪酶效果。实验结果将为进一步研究龙牙百合多糖的构效关系和开发具有潜力的功能性食品提供依据，并为百合的应用提供理论参考。

Research progress on pharmacological effects of lily polysaccharide

Lilium brownii var. viridulum is one of the main traditional edible and medicinal lily in China. This study aimed to explore the extraction technology, physicochemical properties and functional activity of polysaccharide from Lilium brownii var. viridulum, used Lilium brownii var. viridulum scales as materials, polysaccharide yield as the index, solid-liquid ratio, extraction time and extraction temperature as factors to optimize the extraction process of lily polysaccharide. Then the polysaccharide was purified through removing protein. The physicochemical properties of the polysaccharides were analyzed, and the antioxidant and lipid-lowering abilities were measured in vitro. The results showed that the optimal extraction conditions were the material to liquid ratio 1:20 g/mL, extraction time 20 min, extraction temperature 75℃, and polysaccharide yield was 11.98%. The contents of total sugar, uronic acid and protein of crude polysaccharide were 58.46%, 8.06% and 10.85%, respectively. After deproteinization, the contents of total sugar and uronic acid of polysaccharide were increased to 84.78% and 15.41%, respectively, and the content of protein was reduced to 4.73%. The scavenging rates of 10 mg/mL deproteinized polysaccharide for DPPH and ABTS⁺ free radicals were 8.75% and 38.59%, respectively, which were lower than those of control V_C. The inhibition rate of deproteinized polysaccharide for pancreatic lipase was 85.78%, which was higher than that of the control orlistat, indicating good inhibit pancreatic lipase effect in vitro. These results would provide a basis for further study on the structure-activity relationship and development of potential functional food, and provide theoretical reference for the application of lily.