番茄红素对长波紫外线照射大鼠皮肤中丙二醛形成和基质金属蛋白酶表达的抑制作用

为了研究了番茄、野樱莓提取物抗皮肤光老化功效和机制，在UVB辐照HaCaT细胞模型上，研究了番茄、野樱莓提取物对细胞凋亡、活性氧（Reactive oxygen species，ROS）、前列腺素E2（Prostaglandin E₂，PGE₂）的影响。在UVB辐照ICR小鼠模型上，通过苏木精-伊红（hematoxylin-eosin，HE）染色评价番茄野樱莓组合物（LA）抵御皮肤光老化作用。研究皮肤组织中超氧化物歧化酶（superoxide dismutase，SOD）活性、丙二醛（Malondialdehyde，MDA）水平以及促炎因子白细胞介素-1β（Interleukin-1β，IL-1β）、白细胞介素-6（Interleukin-6，IL-6）、环氧化酶-2（Cyclooxygenase-2，COX-2）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的表达变化。结果表明，番茄提取物和野樱莓提取物对HaCaT不存在细胞毒性。在HaCaT细胞光老化模型中，与模型组相比，100 μg/mL番茄提取物组和30 μg/mL野樱莓提取物组凋亡细胞数量显著减少（P<0.05）。100 μg/mL野樱莓提取物能够显著降低ROS水平（P<0.01）。100 μg/mL浓度下，野樱莓提取物和番茄提取物组都能够显著降低PGE₂（P<0.05）。与模型组相比，口服LA能够使真皮厚度显著降低（P<0.01），皮肤组织中SOD活性显著增加（P<0.01），MDA水平显著降低（P<0.01），促炎因子IL-6、COX-2、TNF-α显著降低（P<0.05）。番茄、野樱莓提取物组合可以改善UVB诱导的氧化应激和炎症反应，具有抗光老化功效。

Molecular mechanisms of UVB-induced senescence of dermal fibroblasts and its relevance for photoaging of the human skin

In order to investigate the anti-photoaging effect and mechanism of tomato and aronia extracts, the effects of tomato extract and aronia extract on apoptosis, reactive oxygen species (ROS) and prostaglandin E₂ (PGE₂) were studied in UVB-irradiated HaCaT cells. Hematoxylin-eosin (HE) staining was used to evaluate the anti-photoaging effect of tomato and aronia complex (LA) on skin photoaging in UVB-irradiated ICR mice. The activity of superoxide dismutase (SOD), the level of malondialdehyde (MDA) and the expression of pro-inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) were detected. The results showed that tomato extract and aronia extract were not cytotoxic to HaCaT cells. In the photoaging model of HaCaT cells, compared with the model group, the number of apoptotic cells in 100 μg/mL tomato extract group and 30μg/mL aronia extract group were significantly decreased (P<0.05). Aronia extract (100 μg/mL) significantly decreased ROS level (P<0.01). At 100 μg/mL, PEG₂ was significantly decreased in both tomato and aronia extract groups (P<0.05). Compared with the model group, LA treatment group resulted in a significant reduction in dermal thickness (P<0.01) and MDA levels (P<0.01), and a significant increase in SOD activity (P<0.01) in skin tissue, while the proinflammatory factors IL-6, COX-2 and TNF-α were all significantly decreased (P<0.05). Tomato and aronia complex can improve oxidative stress and inflammation induced by UVB, and has the effect of anti-photoaging.