淡豆豉中多糖的分离纯化及结构特征研究

以中药淡豆豉为原料,采用超声法提取淡豆鼓多糖,硫酸-苯酚法检测总多糖含量。通过透析、醇沉、酶解蛋白、H2O2脱色,并经Sephadex G-100凝胶柱层析进一步纯化,得到多糖组分SPSS和SPSSII。利用凝胶渗透色谱(GPC)对其进行相对分子质量分析,红外光谱检测主要结构特征。结果表明,淡豆豉中总多糖含量为0.91%,SPSSI和SPSSII为均一性多糖,相对分子质量分别约为9500和7000,其结构具有多糖类物质的典型特征。

Isolation,Purification and Structure Chareacterization of Polysaccharides from Semen Sojae Preparatum

Polysaccharides were extracted using ultrasonic from the raw material of Semen Sojae Preparatum.The total content of polysaccharides was detected with a UV spectrophotometer after colcration by sulphuric acid and phenylic acid.After dialyzing,depositing with alcohol,enzymolysising the protein with pepsin and decolring by H_2O_2 the polysaccharides were further purified by Sephadex G-100 column chromatography,and then SPSS I and SPSS II were obtained.The relative molecular mass of SPSS I and SPSS II was analyzed respectively by the method of gel-permeation chromatography(GPC),and their structure was characterized by IR spectroscopy.The experiments indicated that the total content of polysaccharides was 0.91% in Semen Sojae Preparatum Both SPSSI and SPSSII were uniform polysaccharide,whose relative molecular mass were about 9500 and 7000 respectively IR spectra showed that both of them had the typical stucture of polysaccharide.