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High Performance Liquid Chromatographic Determination of Phytic Acid in Foods
Authors:KEN LEE  J. A. ABENDROTH
Affiliation:Authors Lee and Abendroth are affiliated with the Dept. of Food Science, Univ. of Wisconsin-Madison, 1605 Linden Drive, Madison, WI 53706.
Abstract:Phytic acid was quantified using a tetrabutylammonium-formate ion pair on an octadecyl column in HPLC. Unlike previous methods, phytic acid eluted significantly later than the dead volume. Good separations were possible with a linear response up to 40 micrograms and a sensitivity of 0.002 micrograms phytic acid. Results with this method are compatible with conventional methods for phytic acid.
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