烤烟基因型间多元有机酸含量差异性分析

为了解烟草不同基因型在不同生态条件下多元有机酸含量差异性,以52个不同基因型烤烟种质在山东和四川两个不同生态区试验得到的原烟样品,利用气相色谱进行了多元有机酸含量检测。检测结果分析表明,苹果酸、草酸和柠檬酸是烤烟各基因型的主要多元有机酸,其中苹果酸含量占多元有机酸总量的75%左右;两产区多元有机酸总量均高于平均值的基因型种质有17份,多为国外引进品种,两产区多元有机酸总量均低于平均值的基因型种质有18份,多为国内育成品种,这35个品种多元有机酸含量遗传相对稳定;四川产区整体上多元有机酸绝对含量较低、相对含量较高,特别是丙二酸、柠檬酸等绝对含量在所有基因型中普遍较低,丁二酸、草酸、苹果酸等相对含量普遍较高,山东产区则与之相反;基因型和生态条件对烤烟多元有机酸含量均有明显影响,相对而言基因型的作用较大。

Comparison of Multiple Organic Acid Contents in Different Flue-cured Tobacco Genotypes

To investigate the difference of multiple organic acid contents in tobacco of different genotypes under two ecological conditions, the multiple organic acid contents in flue-cured tobacco of 52 genotypes from Zhucheng in Shandong and Xichang in Sichuan were determined by gas chromatography. The results showed that malic acid, oxalic acid and citric acid were the major multiple organic acids, especially malic acid which accounted for about 75% of the total amount of multiple organic acids. There were 17 fluecured tobacco varieties whose multiple organic acid contents were higher than the average, most of which were foreign varieties. Most of the 18 flue-cured tobacco varieties with multiple organic acid content lower than the average were internal varieties. The multiple organic acid contents of 35 flue-cured tobacco varieties had good genetic stability. Overall, the absolute contents of multiple organic acids from Sichuan were low and relative contents were high. In particular, the absolute contents of malonic acid, citric acid were low and the relative contents of oxalic acid, malic acid, succinic acid were high for all the tested varieties. Shandong was the opposite of Sichuan. Both genotype and ecological condition significantly affected the multiple organic acid contents in flue-cured tobacco, but the effect of genotype was relatively higher.