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Enrichment of salmon oil with n‐3 PUFA by lipolysis,filtration and enzymatic re‐esterification
Authors:Michel Linder  Excellent Matouba  Jacques Fanni  Michel Parmentier
Abstract:Selective enzymatic hydrolysis of salmon oil extracted without solvent from by‐products was carried out under mild conditions, using a stereospecific sn‐1, sn‐3 lipase Novozyme®. A modification of the lipid class composition was obtained by controlling the degree of hydrolysis (40%, 24 h). The mixture of acylglycerols and free fatty acids was submitted to a filtration step to retain in the retentate most of the saturated fatty acids, with melting peaks ranging from ‐31.9 °C to +14.7 °C obtained by differential scanning calorimetry. This step allowed a significant increase of polyunsaturated fatty acids (PUFA) from 39.2 mol‐% in the crude oil to 43.3% in the permeate. The remaining free fatty acids in the permeate (20.2 wt‐%) was re‐esterified with an immobilized 1, 3‐specific lipase IM60. Acylglycerols synthesis reached 90% in optimized conditions. After 48 h of reaction, the distribution of monoacylglycerols, diacylglycerols and triacylglycerols was 22.1, 28.7, 43.4 (w/w), respectively. The re‐esterification step did not modify the PUFA content obtained after membrane filtration.
Keywords:salmon oil  hydrolysis  lipase  membrane separation  PUFA  DSC
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