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Degradation of a Foam‐Promoting Barley Protein by a Proteinase from Brewing Yeast
Authors:Rena Leisegang  Ulf Stahl
Abstract:The degradation of a major protein component in beer, the lipid transfer protein (LTP1), by the yeast proteinase A was determined. Another major protein fraction in beer, the protein Z‐fraction, was not degraded by this enzyme. Protein preparations from beer and barley containing LTP1 were examined for degradation by proteinase A using SDS‐PAGE, immunoblotting and RP‐HPLC. LTP1 from barley was completely resistant to proteinase A, whereas LTP1 concentrated from beer was cleaved. We conclude that LTP1 was modified during the brewing process, thus rendering it more susceptible to proteinase A degradation.
Keywords:Barley  beer  foam  lipid transfer protein  LTP1  proteinase A
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