| 啤酒污染乳酸菌PCR引物的设计 |
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| 引用本文： | 郑飞云,金建中,顾国贤.啤酒污染乳酸菌PCR引物的设计[J].酿酒,2002,29(2):44-47. |
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| 作者姓名： | 郑飞云 金建中 顾国贤 |
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| 作者单位： | 1. 江南大学酿酒科学系,江苏,无锡,214036
2. 复旦大学遗传研究所国家重点实验室,上海,200433 |
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| 摘 要： | 根据细菌16s rDNA序列的特点，通过对啤酒污染菌16s rDNA序列进行分析，设计合成了针对啤酒污染乳酸菌的引物，在16srDNA基因水平证明了该引物对乳酸菌的特异性，该引物的特异性是PCR检测技术在啤酒厂推广应用的前提，同时反映了16s rDNA在微生物鉴定中所起的重要作用。
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| 关 键 词： | 啤酒 污染乳酸菌 引物 设计 16srDNA PCR检测 |
| 文章编号： | 1002-8110(2002)02-0044-04 |
| 修稿时间： | 2002年1月8日 |
The PCR Primers Design Of Beer Spoilage Lactic Acid Bacteria |
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| ZHENG Fei-yun ,JIN Jian-zhong ,GU Guo-xian.The PCR Primers Design Of Beer Spoilage Lactic Acid Bacteria[J].Liquor Making,2002,29(2):44-47. |
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| Authors: | ZHENG Fei-yun JIN Jian-zhong GU Guo-xian |
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| Affiliation: | ZHENG Fei-yun 1,JIN Jian-zhong 2,GU Guo-xian 1 |
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| Abstract: | After studying the characteristic of the bacteria 16s ribosomal RNA gene (16s rDNA ) and analyzing their sequences of beer spoilage bacteria(LAB) , a pair of lactic acid bacteria primers were designed 16s rDNA showed that the primers were special ones of LAB Using the primers, LAB was detected by polymerase chain reaction (PCR) in brewery This study also showed that 16s rDNA was important to identify microorganism |
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| Keywords: | ribosome 16s rDNA beer lactic acid bacteria |
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