| H2O2对DNA影响的拉曼光谱分析 |
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| 引用本文： | 周殿凤,柯惟中,衡航,籍康. H2O2对DNA影响的拉曼光谱分析[J]. 南京师范大学学报, 2006, 6(3): 83-85 |
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| 作者姓名： | 周殿凤 柯惟中 衡航 籍康 |
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| 作者单位： | 盐城师范学院,物理系,江苏,盐城,224002;江苏省光电技术重点实验室,南京师范大学分析测试中心,江苏,南京,210097 |
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| 摘 要： | 检测了鲱精DNA水溶液及其经过1%、2%和3%H2O2处理48h的拉曼光谱图.实验结果表明DNA在水溶液中同时具有A、B两种构象,但以B型构象为主.经H2O2处理后,DNA水溶液的A型构象有所增强,碱基堆积程度下降.H2O2对碱基和脱氧核糖几乎没有损伤,对DNA的主链结构也只造成很轻微的损伤.由此可见H2O2本身对DNA的损伤是很小的,只有在外界因素诱发下H2O2经反应生成HO.才会给DNA造成严重损伤.
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| 关 键 词： | 拉曼光谱 DNA H2O2 |
| 文章编号： | 1672-1292（2006）03-0083-03 |
| 收稿时间： | 2006-01-13 |
| 修稿时间： | 2006-01-13 |
Influence of H2O2 on DNA by Raman Spectroscopy |
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| ZHOU Dianfeng,KE Weizhong,HENG Hang,JI Kang. Influence of H2O2 on DNA by Raman Spectroscopy[J]. Journal of Nanjing Nor Univ: Eng and Technol, 2006, 6(3): 83-85 |
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| Authors: | ZHOU Dianfeng KE Weizhong HENG Hang JI Kang |
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| Abstract: | The Raman spectra of DNA in aqueous solution treated by 1wt.-%,2wt.-% and 3wt.-% H_2O_2for 48h are measured.The Raman spectral measurements indicate that DNA in aqueous solution shows both A-type and B-type conformation with B-type conformation in the majority.A-type conformation of DNA in aqueous solution is dominated after treatment of H_2O_2 for 48h.H_2O_2 leads to decrease in the base stacking.H_2O_2doesn't damage the bases nearly,and only damages the backbone of DNA slightly.The damage of DNA is mainly from HO resulted from chemical reaction instead of H_2O_2 itself. |
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| Keywords: | raman spectra DNA H_2O_2 |
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