添加有机酸加速2,4,6-三氯酚的生物降解

2,4,6-三氯酚（TCP）属于难生物降解有机物,但通过添加易于生物降解的有机酸可以加速其生物降解的速率.本实验通过添加甲酸和乙酸来研究其对提高TCP生物降解速率影响.同样加入1 mM的甲酸和乙酸可以分别提高TCP生物降解速率66％和88％.对TCP代谢途径分析发现,TCP在生物降解过程中,通过分子氧和电子载体的共基质作用启动其初始的还原和单加氧反应,在苯环上加入羟基.不同加入量的甲酸和乙酸对TCP降解的实验表明,加入0.25mM的乙酸加速TCP生物降解速率正好与加入1mM甲酸的效果相当.这表明,电子数的加入对TCP的生物降解成正比.

Accelerating 2,4,6-trichlorophenol biodegradation by adding organic acid

2,4,6-trichlorophenol （TCP） belongs to one of recalcitrant organic compounds, but its biodegrada- tion could be accelerated by adding readily biodegradable Readily Biodegradable Readily Biodegradable car- boxylic acids. In this work, TCP removal rates were accelerated at different degree by adding formic acid and acetic acid into the TCP solution, and TCP removal rates were increased by 66% and 88% by adding 1 mM formic acid and 1 mM acetic acid, because acetic acid has as 4 times electron equivalents as formic acid has. TCP biodegradation pathway suggested that biotransformation is initiated by a reduction and a mono-oxygena- tion reaction that require molecular oxygen （O2） and an intracellular electron carrier （ represented as 2H） and insert - OH groups into the ring during TCP biodegradation. The experiments with different amount of formic acid and acetic acid added document that 0.25 raM acetic acid and 1 mM formic acid had the same effect on TCP removal rates, which suggested that amount of electron equivalents is proportional to TCP removal rates.