突变型P53特异性活化T细胞的诱导与扩增

采用多种细胞因子诱导外周血单核细胞分化为成熟的树突状细胞（DC,Dentritic Cell）,将成熟DC与初始T细胞共培养,并连续加入DC刺激从而避免活化的T细胞凋亡。将活化的T细胞转入包被有CD3和CD28抗体的板中,使活化T细胞进一步大量扩增。流式检测多因子诱导后DC表型发现,高表达CD83、CD11c、CD11b、CD80、CD86、CCR7、CD54,低表达CD14、CD3、CD33,说明因子成功诱导单核细胞为成熟DC。流式检测扩增的活化T细胞发现高表达CD3、CD8,含有很低量的调节性T细胞（Treg）,分泌大量的IFN-γ,表明扩增出的细胞中活化T的含量很高,为临床研究提供参考。

Induction and Amplification of Mutant P53 Specific Activated T Cells

This study induces peripheral blood mononuclear cells to differentiate into mature dentritic cell（DC）by using multiple cell factors,co-cultures mature DC and original T cell,adds DC stimulation continuously to avoid activated T cell apoptosis and transfers activated T cell to a plate coated with CD3 and CD28so that activated T cell can be amplified further.Flow test on DC phenotype subject to multi-factor induction finds CD83,CD11 c,CD11b,CD80,CD86,CCR7 and CD54with high expression and CD14,CD3 and CD33with low expression,indicating that factors successfully induce mononuclear cells into mature DC.Flow test on amplified activated T cell finds CD3 and CD8with high expression which contain a small amount of regulatory T cells（Treg）and secrete a lot of IFN-γ,indicating that amplified cells have a high content of activated T.This provides reference for clinical research.