| 家蚕杆状病毒表面展示SARS-CoV的RBD蛋白及其免疫原性研究 |
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| 引用本文： | 闫晶晶,;张耀洲,;陈剑清,;于威,;舒特俊.家蚕杆状病毒表面展示SARS-CoV的RBD蛋白及其免疫原性研究[J].浙江工程学院学报,2014(6):710-715. |
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| 作者姓名： | 闫晶晶 ;张耀洲 ;陈剑清 ;于威 ;舒特俊 |
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| 作者单位： | [1]浙江理工大学生命科学学院,杭州310018; [2]天津国际生物医药联合研究院家蚕生物反应器制药平台,天津300457; [3]天津大学药物科学与技术学院,天津300072 |
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| 基金项目： | 国家高技术发展计划“863”项目（2011AA100603）;浙江省自然科学基金面上项目（207217） |
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| 摘 要： | 利用杆状病毒表面展示技术,构建展示SARS-CoV的棘突蛋白（spike protein）受体结合结构域（receptor binding domain,RBD）的重组杆状病毒vBmgp64-RBD,并进一步研究其展示目的蛋白的免疫原性。结果表明：RBD蛋白正确表达并成功展示在病毒囊膜表面,将灭活的纯重组杆状病毒vBmgp64-RBD皮下注射BALB/c小鼠能产生抗RBD蛋白的特异性抗体,且抗体具有抗RBD蛋白的中和作用。证明vBmgp64-RBD有可能作为一种基于杆状病毒的新型SARS疫苗,为SARS的体外检测及后续疫苗的研究开发奠定了基础。
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| 关 键 词： | 杆状病毒表面展示技术 SARS-CoV RBD蛋白 |
Study on Silkworm Baculovirus Surface Display of RBD Protein of SARS-CoV and Its Immunogenicity |
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| Affiliation: | YAN Jing-jing,ZHANG Yao-zhou,CHEN Jian-qing,YU Wei,SHU Te-jun(1. School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2. Silkworm Bioreactor Pharmaceutical Platform, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China; 3. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China) |
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| Abstract: | This study establishes recombinant baculovirus vBmgp64-RBD displaying spike protein receptor binding domain（RBD）of SARS-CoV through baculovirus surface display technique and further studies the immunogenicity of its target protein.The result shows that RBD protein is correctly expressed and successfully displayed on the surface of virus cyst membrane.BALB/c mice with subcutaneous injection of inactivated pure baculovirus vBmgp64-RBD can produce anti-RBD protein specific antibody which has anti-RBD protein neutralization.It proves that vBmgp64-RBD might be used as a new SARS vaccine based on baculovirus.This study lays a foundation for in vitro test of SARS and research and development of subsequent vaccines. |
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| Keywords: | baculovirus surface display technique SARS-CoV RBD protein |
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